Today, I plan to stain the cut glass, glass vessels and T25 Flasks.

Before I head over to the lab, I always review the protocol and make sure I have an easily accessible copy.  While it is simple, I have not done it often enough to remember the process without error.

BASIC H&E STAIN:

1. Remove PBS
2. Add Hematoxylin – leave for 5 min
3. Rinse under running water
4. Add Ammoniated water for 30sec (2 – 3 drops ammonia  to 400mL distilled water)
5. Rinse under running water
6. Add Eosin for 2 min
7. Rinse under running water
8. Add 95% Etoh for 30sec with agitation
9. Add 100% Etoh wash x 3

I still need to check with the lab manager if I they are happy that I preserve the stained cells in resin for removal from the lab.

Reviewing the protocol also ensures that I check materials prior to starting the process – there is nothing worse than starting a protocol only to discover that some of the materials are missing.