It’s passage time again + bonus clones

We’ve continued with our standard schedule which takes us to our regular Thursday passage time. I am happy to report that my plates are looking much better than documented previously:

SK iPSC 19/05/22
Light microscope image of iPSC colony on 19/05/22
SK iPSC 19/05/22
Light microscope image of iPSC colony on 19/05/22
SK iPSC 19/05/22
Microscope image of iPSC colonies ready for passage on 19/05/22

Since I have nice separated colonies, Ash suggested that I select single colonies from two plates to establish two clones (1.1 and 1.2) in separate dishes along with a third mixed (3 x colony) plate. This feels like another milestone.

Even my gridding technique has improved 🙂

SK iPSC 19/05/22
iPSC colony gridded as part of passage protocol on 19/05/22
SK iPSC 19/05/22
Gridded cell sections lifted for transfer to new Petri dish on 19/05/22

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