The Poly-L-Lysine coating has worked well to encourage growth of HBVPs on the base surface of the glass Petri dishes. They look comparable to the T75 Flask cells.
HBVPs growing on base of glass Petri Dish (no engraving) coated with PLL 13/9/21
HBVPs growing in T75 Flask – 13/9/21
Cells are also growing well in the engraved Petri dishes although it is hard to see the cells on the engraved surface.
HBVPs growing on base of glass Petri Dish (with engraving) coated with PLL 13/9/21
To better get a sense of where the cells are growing, we will fix them with 4% Paraformaldehyde (PFA) and then stain them with hematoxylin and eosin (H&E). Hematoxylin has blue-purple color and stains cell nuclei. Eosin is pink and stains proteins more generally.