While I wait for ethics clearance and team members to return to the lab, I am continuing to culture my cells.

3 x T75 and 1 x Cut Glass Dish ready for cell maintenance (media change) on 21/1/22.

At this point, I am feeding more confluent cells on a weekly basis (depending on how they look):

T75 originally plated 15/12/21 – Flask #1

T75 originally plated 15/12/21 – Flask #2

T75 originally plated 7/10/21

Cut Glass Dish – originally plated 15/12/21

While the cut glass dish is hard to image due to the more uneven surface of the glass, and being contained in a larger Petri Dish, there seems to be a good level of cell growth. Perhaps this will result in better staining in the next round.

I still have four other T75s. However, since these are less dense in cell numbers, I am limiting media change to reduce ongoing maintenance costs and media usage:

T75 originally plated 7/10/21 and passaged 16/11/21 – Flask #1

T75 originally plated 7/10/21 and passaged 16/11/21 – Flask #2

T75 originally plated 15/12/21 – Flask #3

I must admit that I am continually amazed that the original flask of cells plated by Jo-Maree in August last year (and passaged a month later) still has viable cells in it. While much of the T75 flask area is pretty sparse with cells, there are some larger clusters including a growing bunch of elongated fibroid-like cells:

T75 originally plated 10/09/21 showing sparse number of cells with evidence of previous cell movement and presence in ‘ghost trails’.

T75 originally plated 10/09/21 showing small cluster of cells with evidence of previous cell movement and presence in ‘ghost trails’.

T75 originally plated 10/09/21 showing larger cluster of fibroid-like cells.

They are easy to miss, but I’ve made a note to monitor their progress and see how they proliferate. Again…it just takes one mutant to get a cell line going!