Tag Archives: Virus

Virus time!

Despite low PBMC cell numbers, due to my ineffective media change, we were able to proceed with the virus addition as scheduled. Ash is confident that we will still get a few colonies.

First we transferred the PBMCs to a new 24-well plate with fresh media. The reprogramming process, as outlined previously, is deceptively simple and involved adding a specific volume of each viral vector (total of 3 vectors) to the PBMC culture. Thankfully, Ash calculated the miniscule amounts required.

The virus must be kept cold. As such, the 3 vials were collected and kept on ice during the transduction process.

Transduction hood set up
Virus vials on ice and in the biosafety hood ready for transduction of PBMCs

After the virus was added, the PBMC culture was placed back in the incubator for 24 hours.