The lab has a great set-up for fluorescence microscopy which makes imaging quick and easy.
You just need to load the well plate into the machine and set up basic imaging parameters. You do need to image both DAPI and Phalloidin stains, but the software merges the images for you.
Fluorescent Images of Fibroid Cells
Simple graphic interface with presets ready to complete fluorescent microscopy.
As discussed in my previous post on immunostaining, the blue dots indicate nuclei and the green structures reveal the cytoskeleton via binding to actin.
DAPI and Fluorescein Phalloidin staining of confluent fibroid cells P4 (although this is potentially misleading as the cells are very slow growing).
Less confluent wells:
DAPI and Fluorescein Phalloidin staining of fibroid cells P4 which enables better visualisation of individual cells.